Ribosomal Proteins. Isolation of Proteins from 50 S Ribosomal Subunits of Escherichia coli
نویسندگان
چکیده
منابع مشابه
Extraction and isolation of individual ribosomal proteins from Escherichia coli.
We have described a new method for the quantitative separation of ribosomal proteins and ribosomal ribonucleic acid. A procedure for the preparation of individual ribosomal proteins by polyacrylamide gel electrophoresis is also described. By the use of gels with smaller pores, at least four of the electrophoretic components from the 30S ribosome can be split into additional protein fractions. B...
متن کاملCluster of genes in Escherichia coli for ribosomal proteins, ribosomal RNA, and RNA polymerase subunits.
The transducing phage lambdarifd18 isolated by Kirschbaum and Konrad [(1973 J. Bacteriol. 116, 517-526] was found to carry structural genes for several 50S ribosomal proteins and 16S and 23S rRNA. It has previously been demonstrated [Kirschbaum & Scaife (1974) Mol. Gen. Genet. 132, 193-201] that this phage carries genes for the DNA-dependent RNA polymerase (nucleosidetriphosphate:RNA nucleotidy...
متن کاملImmunological similarities between specific chloroplast ribosomal proteins from Chlamydomonas reinhardtii and ribosomal proteins from Escherichia coli.
Polyclonal antibodies were elicited against seven of the 33 different proteins of the large subunit of the chloroplast ribosome from Chlamydomonas reinhardtii. Three of these proteins are synthesized in the chloroplast and four are made in the cytoplasm and imported. In western blots, six of the seven antisera are monospecific for their respective large subunit ribosomal proteins, and none of t...
متن کاملMethylation of ribosomal proteins in Escherichia coli.
Escherichia coli was grown in a medium containing [1-(14)C]methionine and [methyl-(3)H]methionine, and the (3)H/(14)C ratio was determined for each of the ribosomal proteins derived from the 70S ribosome. Evidence indicates that six proteins from the 50S subunit were methylated: L7, L9, L11, L12, L18, and L33. Methylation of several other 50S proteins (such as L1, L3, L5, etc.) may also occur. ...
متن کاملRibosomal components from Escherichia coli 50 S subunits involved in the reconstitution of peptidyltransferase activity.
Incubation of 50 S subunits from Escherichia coli in the presence of 1.5 M LiCl yielded 1 . 5 ~ core particles which contained -20 proteins and were inactive with respect to peptidyltransferase activity. The proteins of the split fraction were isolated and their effects with respect to restoration of activity were tested in reconstitution assays. Purified L16 was able to restore activity, but t...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1971
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1971.tb01585.x